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Alternatively, the stock lifestyle organisms can be developed in an acceptable liquid medium (i.e., SoybeanCasein Digest Broth or Sabouraud Dextrose Broth) and also the cells harvested by centrifugation, then washed and resuspended in sterile saline TS to obtain a microbial count of about 1 × 108 cfu for every mL. [ObserveThe estimate
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